ELISA showing Anti-Lambda Light Chain Antibody [RM127] reacts only to the lambda light chain of human immunoglobulins, and not to the kappa light chain, mouse IgG, rat IgG, or goat IgG.
A titer ELISA using Anti-Lambda Light Chain Antibody [RM127]. The plate was coated with different amounts of human IgG3?. A serial dilution of Anti-Lambda Light Chain Antibody [RM127] was used as the primary antibody, followed by an alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA showing Anti-Lambda Light Chain Antibody [RM127] does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of Anti-Lambda Light Chain Antibody [RM127] and a monkey IgG binding antibody (positive control) was used as the detection antibody.
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