Anti-L1CAM Antibody (A16229)

Name: Anti-L1CAM Antibody
Brand: Antibodies.com
SKU: A16229
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to L1CAM for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-L1CAM Antibody

Description

Rabbit polyclonal antibody to L1CAM.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human CD171/L1CAM (NP_000416.1).

Sequence

IKRSKGGKYSVKDKEDTQVDSEARPMKDETFGEYRSLESDNEEKAFGSSQPSLNGDIKPLGSDDSLADYGGSVDVQFNEDGSFIGQYSGKKEKEAAGGNDSSGATSPINPAVALE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

85 kDa / 200 - 250 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen identified by monoclonal, CAML1, CD171, CD171 antigen, HSAS, HSAS1, Hyd, L1, L1 cell adhesion molecule, L1-NCAM, L1CAM_HUMAN, MASA, MIC5, N CAML1, N-CAM-L1, NCAM-L1, NCAML1, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, NILE, OTTHUMP00000025992, S10, SPG1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-L1CAM Antibody (A16229)

Western blot analysis of extracts of various cell lines, using Anti-L1CAM Antibody (A16229) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Immunofluorescence - Anti-L1CAM Antibody (A16229)

Immunofluorescence analysis of HeLa cells using Anti-L1CAM Antibody (A16229) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 3: Immunofluorescence - Anti-L1CAM Antibody (A16229)

Immunofluorescence analysis of Neuro-2a cells using Anti-L1CAM Antibody (A16229) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Immunofluorescence - Anti-L1CAM Antibody (A16229)

Immunofluorescence analysis of SH-SY5Y cells using Anti-L1CAM Antibody (A16229) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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