This antibody reacts with Ku80, a 80 kDa subunit of Ku autoantigen (heterodimer of 72 and 87 kDa polypeptides). Ku autoantigen is involved in DNA repair and in V(D)J recombination through its ability to bind DNA double-strand breaks.
Applications
ICC, IP
Dilutions
IHC: 2 µg/ml, PFA-fixation possible.
Reactivity
Human
Immunogen
Human whole T-lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-54
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
80 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunofluorescence staining of HeLa human cervix carcinoma cell line using Anti-Ku80 Antigen Antibody (A85990) (detection by Goat Anti-Mouse IgG2a Alexa Fluor® 488 | green). Actin (red) was stained with Phalloidin-TRITC, nuclei with DAPI (blue).
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