+1 (314) 370-6046 or
Contact Us
Explore our End of Year Promotion >>
Antibodies.com LogoAntibodies.com Logo
Sign In / RegisterMy Account
0

Ku70 (phospho Ser5) Cell Based ELISA Kit (A102676)

Western Blot - Ku70 (phospho Ser5) Cell Based ELISA Kit (CBP1135) - Antibodies.com Protocol Diagram - Ku70 (phospho Ser5) Cell Based ELISA Kit (CBP1135) - Antibodies.com Protocol Summary - Ku70 (phospho Ser5) Cell Based ELISA Kit (CBP1135) - Antibodies.com Western Blot - Control Antibody - Ku70 (phospho Ser5) Cell Based ELISA Kit (CBP1135) - Antibodies.com
$645
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Ku70 (phospho Ser5) Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This Ku70 (phospho Ser5) Cell Based ELISA Kit allows for the detection of Ku70 (phospho Ser5) and the effects that certain stimulation conditions have on Ku70 (phospho Ser5) expression in different cell lines. Qualitative determination of Ku70 (phospho Ser5) concentration is achieved by an indirect ELISA format. In essence, the Ku70 (phospho Ser5) is captured by Anti-Ku70 (phospho Ser5) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Ku70 (phospho Ser5) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Ku70 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
5''-deoxyribose-5-phosphate lyase Ku70, 5''-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 1, ATP dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, Ku 70, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa, Ku p70, Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa, Kup70, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen, Thyroid autoantigen 70kD (Ku antigen), Thyroid lupus autoantigen, Thyroid lupus autoantigen p70, Thyroid-lupus autoantigen, TLAA, X ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair cross-complementing protein 6, XRCC 6, Xrcc6, XRCC6_HUMAN
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Ku70 (phospho Ser5) Cell Based ELISA Kit (CBP1135) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Ku70 (phospho Ser5) Cell Based ELISA Kit (A102676)
Western blot analysis of extracts from HeLa cells, using Anti-Ku70 (phopho Ser5) Antibody.
Protocol Diagram - Ku70 (phospho Ser5) Cell Based ELISA Kit (CBP1135) - Antibodies.comEnlarge Image
Figure 2: Ku70 (phospho Ser5) Cell Based ELISA Kit (A102676)
A diagram showing the protocol for Ku70 (phospho Ser5) Cell Based ELISA Kit.
Protocol Summary - Ku70 (phospho Ser5) Cell Based ELISA Kit (CBP1135) - Antibodies.comEnlarge Image
Figure 3: Ku70 (phospho Ser5) Cell Based ELISA Kit (A102676)
A summary of the protocol for Ku70 (phospho Ser5) Cell Based ELISA Kit.
Western Blot - Control Antibody - Ku70 (phospho Ser5) Cell Based ELISA Kit (CBP1135) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Ku70 (phospho Ser5) Cell Based ELISA Kit (A102676)
The mouse monoclonal antibody to GAPDH is used as a positive control inKu70 (phospho Ser5) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Publishing research using Ku70 (phospho Ser5) Cell Based ELISA Kit (A102676)? Please let us know so that we can list the citation on this page.
Main Menu Contact Us 0Checkout
Top