Anti-Ku70 Antibody (A15646)

Name: Anti-Ku70 Antibody
Brand: Antibodies.com
SKU: A15646
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Ku70 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-Ku70 Antibody

Description

Rabbit polyclonal antibody to Ku70.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:100, ICC/IF: 1:50-1:100, IP: 1:20-1:50

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human Ku70 (NP_001460.1).

Sequence

PEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

70 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

5''-deoxyribose-5-phosphate lyase Ku70, 5''-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP dependent DNA helicase 2 subunit 1, ATP dependent DNA helicase II 70 kDa subunit, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6, G22P1, Ku 70, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa, Ku p70, Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa, Kup70, Lupus Ku autoantigen protein p70, ML8, Thyroid autoantigen, Thyroid autoantigen 70kD (Ku antigen), Thyroid lupus autoantigen, Thyroid lupus autoantigen p70, Thyroid-lupus autoantigen, TLAA, X ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair complementing defective repair in Chinese hamster cells 6, X-ray repair cross-complementing protein 6, XRCC 6, Xrcc6, XRCC6_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-Ku70 Antibody (A15646)

Western blot analysis of extracts of various cell lines, using Anti-Ku70 Antibody (A15646) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Ku70 Antibody (A15646)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-Ku70 Antibody (A15646) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Ku70 Antibody (A15646)

Immunohistochemistry analysis of paraffin-embedded human gastric cancer using Anti-Ku70 Antibody (A15646) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Ku70 Antibody (A15646)

Immunohistochemistry analysis of paraffin-embedded human uterus using Anti-Ku70 Antibody (A15646) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.