Anti-KRAS (phospho Ser89) Antibody (A308919)

Western blot analysis of extracts of various cell lines, using Anti-KRAS (phospho Ser89) Antibody (A308919) at 1:500 dilution. Jurkat cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. NIH/3T3 cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-KRAS (phospho Ser89) Antibody (A308919) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-KRAS (phospho Ser89) Antibody (A308919) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.