Anti-KLF4 Antibody (A15314)

Name: Anti-KLF4 Antibody
Brand: Antibodies.com
SKU: A15314
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to KLF4 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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iPSCs Marker

Specifications

Name

Anti-KLF4 Antibody

Description

Rabbit polyclonal antibody to KLF4.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 416-513 of KLF4 (NP_001300981.1).

Sequence

KRGRRSWPRKRTATHTCDYAGCGKTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDRAFSRSDHLALHMKRHF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

62 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Endothelial Kruppel like zinc finger protein, Epithelial zinc finger protein EZF, EZF, GKLF, gut Kruppel-like factor, Gut-enriched krueppel-like factor, KLF, KLF4_HUMAN, Krueppel-like factor 4, Kruppel like factor 4 (Epithelial zinc finger protein EZF) (Gut enriched Krueppel like factor), Kruppel like factor 4 (gut)

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-KLF4 Antibody (A15314)

Western blot analysis of various lysates, using Anti-KLF4 Antibody (A15314) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-KLF4 Antibody (A15314)

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Figure 3: Immunohistochemistry - Anti-KLF4 Antibody (A15314)

Immunohistochemistry analysis of paraffin-embedded human stomach cancer using Anti-KLF4 Antibody (A15314) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-KLF4 Antibody (A15314)

Immunofluorescence analysis of U2OS cells using Anti-KLF4 Antibody (A15314) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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