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Anti-Kir4.1 Antibody (A305164)

Immunohistochemistry - Anti-Kir4.1 Antibody (A305164) - Antibodies.com Western Blot - Anti-Kir4.1 Antibody (A305164) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-Kir4.1 Antibody (A305164) - Antibodies.com
$500
Product Datasheet

Rabbit polyclonal antibody to Kir4.1 for WB, ICC/IF and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-Kir4.1 Antibody
Description
Rabbit polyclonal antibody to Kir4.1.
Applications
WB, ICC/IF, IHC
Dilutions
WB: 1:1,000, ICC/IF: 1:100, IHC: 1:50
Reactivity
Human, Rat
Immunogen
Synthetic peptide from the mid-protein of Human Kir4.1.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
~42 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ATP dependent inwardly rectifying potassium channel Kir4.1, ATP sensitive inward rectifier potassium channel 10, ATP-dependent inwardly rectifying potassium channel Kir4.1, ATP-sensitive inward rectifier potassium channel 10, BIRK10, Glial ATP dependent inwardly rectifying potassium channel KIR4.1, Inward rectifier K(+) channel Kir1.2, Inward rectifier K+ channel KIR1.2, Inwardly rectifying potassium channel Kir1.2, inwardly rectifying subfamily J member 10, KCJ10_HUMAN, KCNJ 10, Kcnj10, KCNJ13 PEN, KIR1.2, Potassium channel, Potassium channel inwardly rectifying subfamily J member 10, Potassium inwardly rectifying channel subfamily J member 10, SESAME
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunohistochemistry - Anti-Kir4.1 Antibody (A305164) - Antibodies.comEnlarge Image
Figure 1: Immunohistochemistry - Anti-Kir4.1 Antibody (A305164)
Immunohistochemistry analysis of human kidney, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-Kir4.1 Antibody (A305164) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western Blot - Anti-Kir4.1 Antibody (A305164) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Kir4.1 Antibody (A305164)
Western blot analysis of rat liver cell lysates showing detection of ~42.5 kDa Kir4.1 protein using Anti-Kir4.1 Antibody (A305164) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~42.5 kDa.
Immunocytochemistry/Immunofluorescence - Anti-Kir4.1 Antibody (A305164) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Kir4.1 Antibody (A305164)
Immunocytochemistry/Immunofluorescence analysis of human colon carcinoma cell line (RKO), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-Kir4.1 Antibody (A305164), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Membrane, Cytoplasm. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Kir4.1 Antibody. (D) Composite.
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