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Primary Antibodies
Anti-KIF4A/KIF4 Antibody (A83849)
This product is identical to Abcam (ab3815). Discover more for less.
Overview
Specifications
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$480
Product Datasheet
Goat polyclonal antibody to KIF4A/KIF4 for ELISA, WB, IF and Flow Cytometry.
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Product Size:
100µg
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Shipping Information
Delivery
Freight Charges
$40 to United States
Lead Time
3-5 Business Days
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Telephone
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Specifications
Name
Anti-KIF4A/KIF4 Antibody
Description
Goat polyclonal antibody to KIF4A/KIF4.
Applications
ELISA
,
WB
,
IF
,
Flow Cytometry
Dilutions
ELISA: 1:64,000, WB: 1-2µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human, Mouse
Immunogen
Synthetic peptide corresponding to the C terminal region of human KIF4A/KIF4.
Sequence
SFFSGCSPIEEEAH
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Molecular Weight
150 kDa
Predicted MW
139.5kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Goat IgG (A121671)
Suitable Secondaries
Donkey Anti-Goat IgG H&L Antibody (AP) (A300679)
Donkey Anti-Goat IgG H&L Antibody (Biotin) (A300716)
Donkey Anti-Goat IgG H&L Antibody (FITC) (A300685)
Donkey Anti-Goat IgG H&L Antibody (HRP) (A300730)
See all Anti-Goat IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Scientific Validation Data (3)
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Figure 1: Western Blot - Anti-KIF4A/KIF4 Antibody (A83849)
Anti-KIF4A Antibody (A83849) (2µg/ml) staining of nuclear NIH3T3 (A) and (2µg/ml) negative control Human Ovary (B) lysate. (35µg protein in RIPA buffer) Detected by chemiluminescence.
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Figure 2: Flow Cytometry - Anti-KIF4A/KIF4 Antibody (A83849)
Anti-KIF4A Antibody (A83849) Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
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Figure 3: Immunofluorescence - Anti-KIF4A/KIF4 Antibody (A83849)
Anti-KIF4A Antibody (A83849) Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/ml) followed by Alexa Fluor 488 secondary antibody (4µg/ml).
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