Anti-KIF3A Antibody (A15313)

Name: Anti-KIF3A Antibody
Brand: Antibodies.com
SKU: A15313
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to KIF3A for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-KIF3A Antibody

Description

Rabbit polyclonal antibody to KIF3A.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:100-1:500, ICC/IF: 1:50-1:200, IP: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 430-699 of human KIF3A (NP_008985.3).

Sequence

RKALETKLDMEEEERNKARAELEKREKDLLKAQQEHQSLLEKLSALEKKVIVGGVDLLAKAEEQEKLLEESNMELEERRKRAEQLRRELEEKEQERLDIEEKYTSLQEEAQGKTKKLKKVWTMLMAAKSEMADLQQEHQREIEGLLENIRQLSRELRLQMLIIDNFIPRDYQEMIENYVHWNEDIGEWQLKCVAYTGNNMRKQTPVPDKKEKDPFEVDLSHVYLAYTEESLRQSLMKLERPRTSKGKARPKTGRRKRSAKPETVIDSLLQ

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

KIF 3, KIF 3A, KIF3, KIF3A_HUMAN, Kifl, Kinesin family member 3A, Kinesin family protein 3, Kinesin family protein 3A, Kinesin like protein KIF 3A, Kinesin like protein KIF3A, Kinesin-like protein KIF3A, Kns3, Microtubule plus end directed kinesin motor 3A, Microtubule plus end-directed kinesin motor 3A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-KIF3A Antibody (A15313)

Western blot analysis of extracts of various cell lines, using Anti-KIF3A Antibody (A15313) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-KIF3A Antibody (A15313)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-KIF3A Antibody (A15313) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-KIF3A Antibody (A15313)

Immunofluorescence analysis of MCF7 cells using Anti-KIF3A Antibody (A15313). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-KIF3A Antibody (A15313)

Immunoprecipitation analysis of 200µg extracts of MCF-7 cells using 3µg of Anti-KIF3A Antibody (A15313). This Western blot was performed on the immunoprecipitate using Anti-KIF3A Antibody (A15313) at a dilution of 1:1000.

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