+1 (314) 370-6046 or
Contact Us
Explore our End of Year Promotion >>
Antibodies.com LogoAntibodies.com Logo
Sign In / RegisterMy Account
0

Keratin 20 Cell Based ELISA Kit (A102895)

Western Blot - Keratin 20 Cell Based ELISA Kit (CB5399) - Antibodies.com Protocol Diagram - Keratin 20 Cell Based ELISA Kit (CB5399) - Antibodies.com Protocol Summary - Keratin 20 Cell Based ELISA Kit (CB5399) - Antibodies.com Western Blot - Control Antibody - Keratin 20 Cell Based ELISA Kit (CB5399) - Antibodies.com
$550
100% Guarantee
Price Match Guarantee
Request a Quotation
Custom or Bulk Request

Freight Charges
$40 to United States
Lead Time
5-8 Business Days

Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Keratin 20 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This Keratin 20 Cell Based ELISA Kit allows for the detection of Keratin 20 and the effects that certain stimulation conditions have on Keratin 20 expression in different cell lines. Qualitative determination of Keratin 20 concentration is achieved by an indirect ELISA format. In essence, the Keratin 20 is captured by Anti-Keratin 20 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Keratin 20 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
CD20, CK 20, CK-20, CK20, Cytokeratin 20, Cytokeratin-20, Cytokeratin20, K1C20_HUMAN, K20, KA20, Keratin, keratin 20, type I, keratin 21, rat, homolog of, Keratin type I cytoskeletal 20, Keratin-20, Keratin20, KRT 20, KRT 21, KRT20, KRT21, MGC35423, OTTHUMP00000164518, Protein IT, type I cytoskeletal 20
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Keratin 20 Cell Based ELISA Kit (CB5399) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Keratin 20 Cell Based ELISA Kit (A102895)
Western blot analysis of extracts from HeLa cells, using Anti-Keratin 20 Antibody.
Protocol Diagram - Keratin 20 Cell Based ELISA Kit (CB5399) - Antibodies.comEnlarge Image
Figure 2: Keratin 20 Cell Based ELISA Kit (A102895)
A diagram showing the protocol for Keratin 20 Cell Based ELISA Kit.
Protocol Summary - Keratin 20 Cell Based ELISA Kit (CB5399) - Antibodies.comEnlarge Image
Figure 3: Keratin 20 Cell Based ELISA Kit (A102895)
A summary of the protocol for Keratin 20 Cell Based ELISA Kit.
Western Blot - Control Antibody - Keratin 20 Cell Based ELISA Kit (CB5399) - Antibodies.comEnlarge Image
Figure 4: Western Blot - Keratin 20 Cell Based ELISA Kit (A102895)
The mouse monoclonal antibody to GAPDH is used as a positive control inKeratin 20 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Publishing research using Keratin 20 Cell Based ELISA Kit (A102895)? Please let us know so that we can list the citation on this page.
Main Menu Contact Us 0Checkout
Top