Synthetic peptide derived from human Keratin 18 around the phosphorylation site of Ser33 (amino acids 1-50).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
47kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CK18, CK19, CK8, K18, K19, K8, keratin 18, keratin 19, keratin 8, Keratin type I 40kDa, Keratin type I cytoskeletal 18, Keratin type I cytoskeletal 19, Keratin type II cytoskeletal 8, KRT18, KRT19, KRT8
Figure 1: Western Blot - Anti-Keratin 18 (phospho Ser33) Antibody (A94573)
Western blot analysis of lysates from HT29 cells using Anti-Keratin 18 (phospho Ser33) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-Keratin 18 (phospho Ser33) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunofluorescence analysis of HeLa cells using Anti-Keratin 18 (phospho Ser33) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast cancer using Anti-Keratin 18 (phospho Ser33) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
