Anti-KCC2 Antibody [S1-12] (A304964)

Name: Anti-KCC2 Antibody [S1-12]
Brand: Antibodies.com
SKU: A304964
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S1-12] antibody to KCC2 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-KCC2 Antibody [S1-12]

Description

Mouse monoclonal [S1-12] antibody to KCC2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:1,000, IHC: 1:300, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein amino acids 932-1043 corresponding to rat KCC2.

Host

Mouse

Clonality

Monoclonal

Clone ID

S1-12

Isotype

IgG2a

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Electroneutral potassium chloride cotransporter 2, Electroneutral potassium-chloride cotransporter 2, Erythroid K Cl cotransporter 2, Furosemide sensitive K Cl cotransporter, hKCC2, K-Cl cotransporter 2, KCC 2, KIAA1176, Neuronal K Cl cotransporter, Neuronal K-Cl cotransporter, Potassium Chloride Cotransporter, Potassium chloride transporter 5, rKCC2, S12A5, S12A5_HUMAN, SLC12A5, Solute carrier family 12 (potassium chloride transporter) member 5, Solute carrier family 12 member 5

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-KCC2 Antibody [S1-12] (A304964)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-KCC2 Antibody [S1-12] (A304964), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) KCC2 Antibody. (D) Composite.

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Figure 2: Western Blot - Anti-KCC2 Antibody [S1-12] (A304964)

Western blot analysis of rat brain membrane lysate showing detection of KCC2 protein using Anti-KCC2 Antibody [S1-12] (A304964) at 1:1,000.

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Figure 3: Immunofluorescence - Anti-KCC2 Antibody [S1-12] (A304964)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-KCC2 Antibody [S1-12] (A304964), at 1:200 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) KCC2 Antibody (D) Composite.

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