Anti-KAP1 (phospho Ser824) Antibody [ARC59356] (A309649)

Name: Anti-KAP1 (phospho Ser824) Antibody [ARC59356]
Brand: Antibodies.com
SKU: A309649
Price: 560.0 USD
Availability: InStock

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$560

Rabbit monoclonal [ARC59356] antibody to KAP1 (phospho Ser824) for WB.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-KAP1 (phospho Ser824) Antibody [ARC59356]

Description

Rabbit monoclonal [ARC59356] antibody to KAP1 (phospho Ser824).

Applications

Dilutions

WB: 1:2,000-1:6,000

Reactivity

Human, Mouse

Immunogen

A synthetic phosphorylated peptide around S824 of human KAP1/TRIM28.

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC59356

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

117 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP-1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TIF1-beta, TIF1B, Transcription intermediary factor 1-beta, TRIM28

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-KAP1 (phospho Ser824) Antibody [ARC59356] (A309649)

Western blot analysis of HeLa, using Anti-KAP1 (phospho Ser824) Antibody [ARC59356] (A309649) at 1:5,000 dilution. HeLa cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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Western Blot - Anti-KAP1 (phospho Ser824) Antibody [ARC59356] (A309649)

Western blot analysis of NIH/3T3, using Anti-KAP1 (phospho Ser824) Antibody [ARC59356] (A309649) at 1:5,000 dilution. NIH/3T3 cells were treated by Etoposide (25 uM) at 37°C for 5 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.

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