Anti-KAP1 Antibody [ARC0047] (A308504)

Name: Anti-KAP1 Antibody [ARC0047]
Brand: Antibodies.com
SKU: A308504
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0047] antibody to KAP1 for WB, IHC and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-KAP1 Antibody [ARC0047]

Description

Rabbit monoclonal [ARC0047] antibody to KAP1.

Applications

WB, IHC, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human KAP1/TRIM28 (Q13263).

Sequence

RNQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFAS

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0047

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

110 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 SUMO protein ligase TRIM28, E3 SUMO-protein ligase TRIM28, FLJ29029, KAP 1, KAP-1, KRAB associated protein 1, KRAB interacting protein 1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP 1, KRIP-1, KRIP1, Nuclear corepressor KAP 1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1-beta, TIF1B, TIF1B_HUMAN, Transcription intermediary factor 1 beta, Transcription intermediary factor 1-beta, Trim28, Tripartite motif containing 28, tripartite motif containing protein 28, Tripartite motif-containing protein 28

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-KAP1 Antibody [ARC0047] (A308504)

Western blot analysis of extracts of various cell lines, using Anti-KAP1 Antibody [ARC0047] (A308504) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Immunohistochemistry - Anti-KAP1 Antibody [ARC0047] (A308504)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-KAP1 Antibody [ARC0047] (A308504) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-KAP1 Antibody [ARC0047] (A308504)

Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-KAP1 Antibody [ARC0047] (A308504) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-KAP1 Antibody [ARC0047] (A308504)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-KAP1 Antibody [ARC0047] (A308504) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.