Synthetic peptide within amino acids 650-700 of human KAP1 (NP_005753. 1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP-1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP-1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TIF1-beta, TIF1B, Transcription intermediary factor 1-beta, TRIM28
Samples Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Anti-KAP1 Antibody (A295397) was used for IP at 6 µg per reaction. KAP1 was also immunoprecipitated by a previous lot of this antibody and another rabbit anti-KAP1 antibody. For blotting immunoprecipitated KAP1, Anti-KAP1 Antibody (A295397) was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Anti-KAP1 Antibody (A295397) was used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
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