Anti-KAP1 Antibody (A13965)

Name: Anti-KAP1 Antibody
Brand: Antibodies.com
SKU: A13965
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to KAP1 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

orders@antibodies.com

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Specifications

Name

Anti-KAP1 Antibody

Description

Rabbit polyclonal antibody to KAP1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:1,000-1:3,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 576-835 of human KAP1/KAP1/TRIM28 (NP_005753.1).

Sequence

PVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

E3 SUMO protein ligase TRIM28, E3 SUMO-protein ligase TRIM28, FLJ29029, KAP 1, KAP-1, KRAB associated protein 1, KRAB interacting protein 1, KRAB-associated protein 1, KRAB-interacting protein 1, KRIP 1, KRIP-1, KRIP1, Nuclear corepressor KAP 1, Nuclear corepressor KAP-1, RING finger protein 96, RNF96, TF1B, TIF1 beta, TIF1-beta, TIF1B, TIF1B_HUMAN, Transcription intermediary factor 1 beta, Transcription intermediary factor 1-beta, Trim28, Tripartite motif containing 28, tripartite motif containing protein 28, Tripartite motif-containing protein 28

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (9)

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Figure 1: Western Blot - Anti-KAP1 Antibody (A13965)

Western blot analysis of extracts of various cell lines, using Anti-KAP1 Antibody (A13965) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-KAP1 Antibody (A13965)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-KAP1 Antibody (A13965) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-KAP1 Antibody (A13965)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-KAP1 Antibody (A13965) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-KAP1 Antibody (A13965)

Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using Anti-KAP1 Antibody (A13965) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.