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Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665)

Western Blot - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) - Antibodies.com Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) - Antibodies.com Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) - Antibodies.com Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) - Antibodies.com
$485
Product Datasheet

Rabbit monoclonal [ARC0811] antibody to K48-linkage specific Ubiquitin for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-K48-linkage specific Ubiquitin Antibody [ARC0811]
Description
Rabbit monoclonal [ARC0811] antibody to K48-linkage specific Ubiquitin.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Ubiquitin (P0CG47).
Sequence
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0811
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
17 - 250 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) - Antibodies.comEnlarge Image

Western Blot - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665)

Western blot analysis of extracts of HeLa cells, using Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) at 1:1,000 dilution. HeLa cells were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665)

Immunofluorescence analysis of C6 cells using Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665)

Immunofluorescence analysis of NIH-3T3 cells using Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665)

Immunofluorescence analysis of U-2 OS cells using Anti-K48-linkage specific Ubiquitin Antibody [ARC0811] (A307665) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
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