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Anti-K11-linkage specific Polyubiquitin Antibody (A309526)

Western Blot - Anti-K11-linkage specific Polyubiquitin Antibody (A309526) - Antibodies.com Dot Blot - Anti-K11-linkage specific Polyubiquitin Antibody (A309526) - Antibodies.com
This product is discontinued

Anti-K11-linkage specific Polyubiquitin Antibody (A309526) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-K11-linkage specific Polyubiquitin Antibody
Description
Rabbit polyclonal antibody to K11-linkage specific Polyubiquitin.
Applications
WB, Dot Blot
Dilutions
DB: 1:500-1:1,000, WB: 1:500-1:2,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K11 linkage).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-K11-linkage specific Polyubiquitin Antibody (A309526) - Antibodies.comEnlarge Image

Western Blot - Anti-K11-linkage specific Polyubiquitin Antibody (A309526)

Western blot analysis of extracts of various cell lines, using Anti-K11-linkage specific Polyubiquitin Antibody (A309526) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
Dot Blot - Anti-K11-linkage specific Polyubiquitin Antibody (A309526) - Antibodies.comEnlarge Image

Dot Blot - Anti-K11-linkage specific Polyubiquitin Antibody (A309526)

Dot blot analysis of a mixture of peptides using Anti-K11-linkage specific Polyubiquitin Antibody (A309526) at a 1:1,000 dilution.