Figure 1: Western Blot - Anti-JunD (phospho Ser255) Antibody [ARC54895] (A305984)
Western blot analysis of extracts of HeLa cells, using Anti-JunD (phospho Ser255) Antibody [ARC54895] (A305984) at 1:2,000 dilution. Hela cells were treated by CIP(20uL/400ul) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
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