Rabbit polyclonal antibody to JunB (phospho Ser259).
Specificity
This antibody detects endogenous levels of JunB only when phosphorylated at Ser259.
Applications
WB, IHC, IP, ELISA
Dilutions
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:20000
Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide derived from human JunB around the phosphorylation site of Ser259 (amino acids 226-275).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
35kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Activator protein 1, AP 1, AP1, Jun B, Jun B proto oncogene, Jun B protooncogene, JunB proto oncogene, JunB protoncogene 9, JunB protooncogene, JUNB_HUMAN, Transcription factor jun B, Transcription factor jun-B, Transcription factor junB
Figure 1: Western Blot - Anti-JunB (phospho Ser259) Antibody (A93637)
Western blot analysis of lysates from HeLa cells using Anti-JunB (phospho Ser259) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-JunB (phospho Ser259) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
![Flow Cytometry - Anti-JunB Antibody [PCRP-JUNB-3G11] (A249094) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249094_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-JunB Antibody [PCRP-JUNB-3G11] - BSA and Azide free (A252274) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252274_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-JunB Antibody [PCRP-JUNB-3G2] (A249095) - Antibodies.com](https://cdn.antibodies.com/image/catalog/249/A249095_1.jpg?profile=product_alternative)
![Flow Cytometry - Anti-JunB Antibody [PCRP-JUNB-3G2] - BSA and Azide free (A252275) - Antibodies.com](https://cdn.antibodies.com/image/catalog/252/A252275_1.jpg?profile=product_alternative)
