Mouse monoclonal [PCRP-JUNB-3G2] antibody to JunB.
Specificity
The c-Jun proto-oncogene was first identified as the cellular homolog of the avian sarcoma virus v-Jun oncogene. The c-Jun protein, along with c-Fos, is a component of the AP-1 transcriptional complex. c-Jun can form either Jun/Jun homodimers or Jun/Fos heterodimers via the leucine repeats in both proteins. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Two additional genes, Jun B and Jun D, have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family.
Activator protein 1, AP 1, AP1, Jun B, Jun B proto oncogene, Jun B protooncogene, JunB proto oncogene, JunB protoncogene 9, JunB protooncogene, JUNB_HUMAN, Transcription factor jun B, Transcription factor jun-B, Transcription factor junB
Flow cytometric analysis of PFA fixed HeLa cells using Anti-JunB Antibody [PCRP-JUNB-3G2] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Unstained cells (red).
Immunofluorescent analysis of PFA fixed HeLa cells stained with Anti-JunB Antibody [PCRP-JUNB-3G2] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Counterstain is Phalloidin (Red).
SDS-PAGE analysis of Anti-JunB Antibody [PCRP-JUNB-3G2] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Analysis of protein array containing more than 19,000 full-length human proteins using Anti-JunB Antibody [PCRP-JUNB-3G2]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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