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Anti-JAK2 (phospho Tyr221) Antibody (A16606)

Western Blot - Anti-JAK2 (phospho Tyr221) Antibody (A16606) - Antibodies.com Immunohistochemistry - Anti-JAK2 (phospho Tyr221) Antibody (A16606) - Antibodies.com
$435
Product Datasheet

Rabbit polyclonal antibody to JAK2 (phospho Tyr221) for WB and IHC.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
Anti-JAK2 (phospho Tyr221) Antibody
Description
Rabbit polyclonal antibody to JAK2 (phospho Tyr221).
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic phosphorylated peptide around Y221 of human JAK2 (NP_004963.1).
Sequence
QDYHI
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
125 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
JAK 2, JAK-2, JAK2_HUMAN, Janus Activating Kinase 2, Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), JTK 10, JTK10, kinase Jak2, OTTHUMP00000043260, THCYT3, Tyrosine protein kinase JAK2, Tyrosine-protein kinase JAK2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-JAK2 (phospho Tyr221) Antibody (A16606) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-JAK2 (phospho Tyr221) Antibody (A16606)
Western blot analysis of extracts of K562 cells, using Anti-JAK2 (phospho Tyr221) Antibody (A16606) at 1:1,000 dilution. K562 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37°C for 1 hour. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-JAK2 (phospho Tyr221) Antibody (A16606) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-JAK2 (phospho Tyr221) Antibody (A16606)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-JAK2 (phospho Tyr221) Antibody (A16606).
Publishing research using Anti-JAK2 (phospho Tyr221) Antibody (A16606)? Please let us know so that we can list the citation on this page.

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