Figure 1: Western Blot - Anti-YTHDF3 Antibody (A16176)
Western blot analysis of extracts of Mouse liver, using Anti-YTHDF3 Antibody (A16176) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Figure 2: Western Blot - Anti-YTHDF3 Antibody (A16176)
Western blot analysis of extracts of HepG2 cells, using Anti-YTHDF3 Antibody (A16176) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-YTHDF3 Antibody (A16176) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-YTHDF3 Antibody (A16176) at a dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells using Anti-YTHDF3 Antibody (A16176) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of NIH/3T3 cells using Anti-YTHDF3 Antibody (A16176) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Figure 7: Western Blot - Anti-YTHDF3 Antibody (A16176)
Immunoprecipitation analysis of 300µg extracts of HT-1080 cells using 3µg of Anti-YTHDF3 Antibody (A16176). This Western blot was performed on the immunoprecipitate using Anti-YTHDF3 Antibody (A16176) at a dilution of 1:1000.
