Anti-Wnt3a Anticorpo (Biotin) (A282269)

Anti-Wnt3a Antibody (Biotin) - BSA and Azide free

Rabbit polyclonal antibody to Wnt3a (Biotin).

This antibody recognises human WNT-3a, a secreted protein and member of the Wnt family of signalling proteins, acting as a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3a plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation. Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a acts upstream of the Notch signalling pathway, in the regulation of somitogenesis, controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways.

WB, ELISA

ELISA: 0.25 - 1 µg/ml, WB: 0.1 - 0.2 µg/ml

Human

Recombinant human WNT-3a.

Rabbit

Polyclonal

IgG

100 µg/ml after reconstitution.

Lyophilized

Reconstitute with 0.5 ml of sterile Phosphate Buffered Saline containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Supplied in Phosphate Buffered Saline.

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Rabbit anti Human WNT-3a antibody recognizes human WNT-3a, a secreted protein and member of the Wnt family of signalling proteins, acting as a ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3a plays key roles in many developmental and cell fate decisions, including embryonic patterning, CNS development, and cytoskeletal formation. Wnt-3a acts as a promoter of the proliferation of ventral midbrain dopaminergic neuron precursor cells, and has been implicated in the progressive differentiation of anterior neural cells, as well as regulating the survival and expansion of neural progenitors derived from human embryonic stem cells. Wnt3a acts upstream of the Notch signalling pathway, in the regulation of somitogenesis, controlled by a segmentation clock, consisting of molecular oscillators in the Notch, Wnt3a and Fgf8 pathways(Aulehla et al. 2003).

Protein Wnt 3a Precursor, Protein Wnt-3a, Wingless type MMTV integration site family member 3A, Wnt 3a, WNT3A protein, WNT3A_HUMAN

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.