Anti-WISP1 Anticorpo (A82877)

Name: Anti-WISP1 Antibody
Brand: Antibodies.com
SKU: A82877
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Novus (NB100-77339). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro WISP1 per ELISA, WB, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-WISP1 Antibody

Descrizione

Goat polyclonal antibody to WISP1.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:128,000, WB: 0.1-0.3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the C terminal region of human WISP1.

Sequenza

C-ESYPDFSEIAN

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

32-35 kDa

MW previsto

30.7kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

CCN 4, CCN family member 4, CCN4, WISP 1, WISP 1c, WISP 1i, WISP 1tc, WISP1c, WISP1i, WISP1tc, Wnt 1 induced secreted protein, WNT 1 induced secreted protein 1, WNT 1 inducible signaling pathway protein 1, Wnt 1 signaling pathway protein 1, Wnt-1 inducible signaling pathway protein 1, Wnt1 induced secreted protein, WNT1 induced secreted protein 1, WNT1 inducible signaling pathway protein 1, Wnt1 signaling pathway protein 1, WNT1-inducible-signaling pathway protein 1

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (5)

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Figure 1: Western Blot - Anti-WISP1 Antibody (A82877)

WISP1 expression in Human Tonsil lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-WISP1 Antibody (A82877) at 0.1µg/ml and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-WISP1 Antibody (A82877)

WISP1 expression in Caco-2 cell lysate 1 (A) and Caco-2 cell lysate 2 (B) + petide (C) analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-WISP1 Antibody (A82877) at 0.3µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-WISP1 Antibody (A82877)

WISP1 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-WISP1 Antibody (A82877) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-WISP1 Antibody (A82877)

WISP1 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-WISP1 Antibody (A82877) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.