Anti-VPS35 Anticorpo [8A3] (A305034)

Western blot analysis of Human, mouse A549, MEF showing detection of VPS35 protein using Anti-VPS35 Antibody [8A3] (A305034) at 1:5 (tissue culture supernatant). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 µg each A549 and MEF. The secondary antibody used was Donkey anti-mouse IRDye 800CW at 1:25,000 in TBS-T.

Western blot analysis of human SH-SY5Y showing detection of VPS35 protein using Anti-VPS35 Antibody [8A3] (A305034) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature.

Immunocytochemistry/Immunofluorescence analysis of human A549 cells, using Anti-VPS35 Antibody [8A3] (A305034), at 1:5 (tissue culture supernatant). The secondary antibody used was Donkey anti-mouse: Alexa Fluor 594 at 1:4,000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells.

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [8A3] (A305034). 500 µL cell culture supernatants were incubated with 10 µL of Protein A/G resin beads for 1 hour at 4°C. A305034 clone 8A3 depletes VPS35 from the A549 cell extract..

Immunohistochemistry analysis of mouse kidney. The Primary Antibody used was Anti-VPS35 Antibody [8A3] (A305034) at 1:100 for Overnight at 4°C, then 30 minutes at 37°C. The secondary antibody used was Goat Anti-Mouse IgG (H+L): FITC for 45 minutes at 37°C. Counterstain: DAPI for 3 min at room temperature. Magnification: 20X.