Anti-VPS35 Anticorpo [7E4] (A305036)

Name: Anti-VPS35 Antibody [7E4]
Brand: Antibodies.com
SKU: A305036
Price: 580.0 USD
Availability: InStock

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$580

Anticorpo monoclonale [7E4] di topo contro VPS35 per WB, ICC/IF e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-VPS35 Antibody [7E4]

Descrizione

Mouse monoclonal [7E4] antibody to VPS35.

Applicazioni

WB, ICC/IF, IP

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:200, IP: 1:200

Reattività

Human, Mouse, Rat

Immunogeno

Full length recombinant human VSP35.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

7E4

Isotipo

IgG2a

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~92 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, hVPS35, Maternal embryonic 3, Maternal-embryonic 3, MEM 3, MEM3, PARK17, TCCCTA00141, Vacuolar protein sorting 35, Vacuolar protein sorting 35 (yeast), Vacuolar protein sorting 35 homolog, Vacuolar protein sorting associated protein 35, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35, VPS 35, VPS35_HUMAN

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (8)

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Figure 1: Immunoprecipitation - Anti-VPS35 Antibody [7E4] (A305036)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [7E4] (A305036) at 1:5 (tissue culture supernatant). 10 ug antibody were coupled to 10 uL A/G resin beads either covalently (with DMP) or non-covalently (1 hour at 4 degrees). The antibody immunoprecipitates VPS35 in mouse and human cells effectively when covalently coupled to the beads..

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Figure 2: Immunoprecipitation - Anti-VPS35 Antibody [7E4] (A305036)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [7E4] (A305036). Three amounts of A305036 (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 lysate for 2 hours at 4°C.

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Figure 3: Immunoprecipitation - Anti-VPS35 Antibody [7E4] (A305036)

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Figure 4: Immunoprecipitation - Anti-VPS35 Antibody [7E4] (A305036)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [7E4] (A305036).

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Figure 5: Immunoprecipitation - Anti-VPS35 Antibody [7E4] (A305036)

Immunoprecipitation analysis of mouse embryonic fibroblast using Anti-VPS35 Antibody [7E4] (A305036). 500 µL cell culture supernatants were incubated with 10 µL of Protein A/G resin beads for 1 hour at 4°C. A305036 clone 7E4 depletes virtually all of the VPS35 from the A549 cell extract..

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Figure 6: Western Blot - Anti-VPS35 Antibody [7E4] (A305036)

Western blot analysis of human SH-SY5Y lysates showing detection of 91.7 kDa VPS35 protein using Anti-VPS35 Antibody [7E4] (A305036) at 1:1,000 for 2 hours at room temperature with shaking. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y. Load: 10 Î¼g. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat anti-mouse IgG:HRP at 1:4,000 for 1 hour at room temperature with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 minutes in room temperature. Predicted/Observed Size: 91.7 kDa.

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Figure 7: Western Blot - Anti-VPS35 Antibody [7E4] (A305036)

Western blot analysis of Human, mouse A549, MEF showing detection of VPS35 protein using Anti-VPS35 Antibody [7E4] (A305036) at 1:5 (tissue culture supernatant). Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells.. Load: 8 µg each A549 and MEF. The secondary antibody used was Donkey anti-mouse IRDye 800CW at 1:25,000 in TBS-T.

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Figure 8: Immunofluorescence - Anti-VPS35 Antibody [7E4] (A305036)

Immunocytochemistry/Immunofluorescence analysis of human A549 cells, using Anti-VPS35 Antibody [7E4] (A305036), at 1:5 (tissue culture supernatant). The secondary antibody used was Donkey anti-mouse: Alexa Fluor 594 at 1:4,000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells.

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