Synthetic peptide from the N-terminal of Human PI 3 Kinase Class 3.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
~101.5 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hVps34, Phosphatidylinositol 3-kinase catalytic subunit type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, PI3-kinase type 3, PI3K type 3, PIK3C3, PtdIns-3-kinase type 3
Immunohistochemistry analysis of human bladder, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-VPS34 Antibody (A304952) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
Western blot analysis of rat, mouse liver cell lysates showing detection of ~101.5 kDa PI 3 Kinase Class 3 protein using Anti-VPS34 Antibody (A304952) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat liver cell lysates. Lane 3: mouse liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~101.5 kDa.
Immunocytochemistry/Immunofluorescence analysis of mouse C2C12 Cells (mouse myoblast cell line), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-VPS34 Antibody (A304952), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Midbody, Late Endosome, Cytoplasmic Vesicle, Autophagosome. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) PI 3 Kinase Class 3 antibody (D) Composite.
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