Anti-Vinculin Antibody [VCL/8533R] - BSA and Azide free
Descrizione
Recombinant rabbit monoclonal [VCL/8533R] antibody to Vinculin.
Specificità
Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including Talin and -Actinin has been shown to link Actin to integrins in the plasma membrane through interactions with the vinculin and Talin complex or by a direct interaction with integrin.
Applicazioni
WB, IHC-P
Controllo positivo
A431, K562, NIH3T3, U-87 or THP-1 cells. Human bladder or testis.
Diluizioni consigliate
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reattività
Human, Mouse
Immunogeno
Recombinant fragment (around Amino Acids 174-322) of human Vinculin (VCL) protein. The exact sequence is proprietary.
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
VCL/8533R
Isotipo
IgG
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
1 mg/ml
Peso molecolare
117 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.