Figure 1: Western Blot - Anti-Vinculin Antibody [ARC51900] (A305534)
Western blot analysis of various lysates, using Anti-Vinculin Antibody [ARC51900] (A305534) at 1:250,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Confocal imaging of HeLa cells using Anti-Vinculin Antibody [ARC51900] (A305534), at a dilution of 1:100, (red). DAPI was used for nuclear staining (Blue). Objective: 60x.
Figure 3: Western Blot - Anti-Vinculin Antibody [ARC51900] (A305534)
Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-Vinculin Antibody [ARC51900] (A305534). This Western blot was performed on the immunoprecipitate using Anti-Vinculin Antibody [ARC51900] (A305534) at a dilution of 1:1000.
Prodotti alternativi a Anti-Vinculin Anticorpo [ARC51900] (A305534)