Recombinant rabbit monoclonal [VIL1/7378R] antibody to Villin.
Specificità
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin.Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Applicazioni
IHC-P
Controllo positivo
A549, HepG2 or HCT116 cells. Human colon or rectum.
Diluizioni consigliate
IHC: 1-2 µg/ml
Reattività
Human
Immunogeno
Recombinant fragment (around Amino Acids 600-700) of human Villin protein. The exact sequence is proprietary.
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
VIL1/7378R
Isotipo
IgG
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A/G chromatography.
Concentrazione
200 µg/ml
Peso molecolare
93 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.