Anti-VGLUT2 Anticorpo [S29-29] (A305012)

Name: Anti-VGLUT2 Antibody [S29-29]
Brand: Antibodies.com
SKU: A305012
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo monoclonale [S29-29] di topo contro VGLUT2 per WB, IHC e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-VGLUT2 Antibody [S29-29]

Descrizione

Mouse monoclonal [S29-29] antibody to VGLUT2.

Applicazioni

WB, IHC, ICC/IF

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat

Immunogeno

Fusion protein amino acids 501-582 (cytoplasmic C-terminus) of rat VGlut2.

Specie ospite

Mouse

Clonalità

Monoclonal

Clona ID

S29-29

Isotipo

IgG1

Coniugare

Unconjugated

Purificazione

Protein G purification.

Concentrazione

1 mg/ml

Peso molecolare

~60 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Differentiation associated BNPI, Differentiation associated Na dependent inorganic phosphate cotransporter, Differentiation associated Na(+) dependent inorganic phosphate cotransporter, Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, DNPI, SLC17A6, Sodium dependent inorganic phosphate cotransporter, Solute carrier family 17 (Sodium dependent inorganic phosphate cotransporter) member 6, Solute carrier family 17 member 6, Vesicular glutamate transporter 2, Vesicular glutamate transporter type 2, VGLU2_HUMAN, VGLUT 2

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Immunofluorescence - Anti-VGLUT2 Antibody [S29-29] (A305012)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-VGLUT2 Antibody [S29-29] (A305012), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGLUT2 Antibody. (D) Composite.

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Figure 2: Western Blot - Anti-VGLUT2 Antibody [S29-29] (A305012)

Western blot analysis of rat brain membrane lysate showing detection of VGLUT2 protein using Anti-VGLUT2 Antibody [S29-29] (A305012) at 1:000.

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Figure 3: Immunofluorescence - Anti-VGLUT2 Antibody [S29-29] (A305012)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-VGLUT2 Antibody [S29-29] (A305012), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT2 Antibody (D) Composite.