This VAV1 (phospho Tyr174) Cell Based ELISA Kit allows for the detection of VAV1 (phospho Tyr174) and the effects that certain stimulation conditions have on VAV1 (phospho Tyr174) expression in different cell lines. Qualitative determination of VAV1 (phospho Tyr174) concentration is achieved by an indirect ELISA format. In essence, the VAV1 (phospho Tyr174) is captured by Anti-VAV1 (phospho Tyr174) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this VAV1 (phospho Tyr174) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-VAV1 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Figure 4: Western Blot - VAV1 (phospho Tyr174) Cell Based ELISA Kit (CBP1089)
The mouse monoclonal antibody to GAPDH is used as a positive control inVAV1 (phospho Tyr174) Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
Prodotti alternativi a VAV1 (phospho Tyr174) Cell Based Kit Elisa (A102273)
