Anti-UVRAG Anticorpo (A305065)

Name: Anti-UVRAG Antibody
Brand: Antibodies.com
SKU: A305065
Price: 500.0 USD
Availability: InStock

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$500

Anticorpo policlonale di coniglio contro UVRAG per WB e ICC/IF.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-UVRAG Antibody

Descrizione

Rabbit polyclonal antibody to UVRAG.

Specificità

Predicted molecular weight at ~78.1 kDa. Observed molecular weights in the 75-90 kDa range.

Applicazioni

WB, ICC/IF

Diluizioni consigliate

WB: 1:1,000, ICC/IF: 1:100

Reattività

Human, Mouse, Rat

Immunogeno

Synthetic peptide from the mid-protein of human UVRAG.

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Peptide affinity purification.

Concentrazione

1 mg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

Beclin 1 binding protein, DHTX, Disrupted in heterotaxy, p63, UV radiation resistance associated, UV radiation resistance associated gene, UV radiation resistance associated gene protein, UV radiation resistance-associated gene protein, UVRAG_HUMAN, VPS38

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-UVRAG Antibody (A305065)

Western blot analysis of rat liver showing detection of ~100kDa UVRAG protein using Anti-UVRAG Antibody (A305065) at 1:200 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: rat liver (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~100kDa.

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Figure 2: Western Blot - Anti-UVRAG Antibody (A305065)

Western blot analysis of human HeLa and HEK293T cell lysates showing detection of ~100kDa UVRAG protein using Anti-UVRAG Antibody (A305065) at 1:200 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human HeLa (20 µg). Lane 3: human 293T (20 µg). Load: 20 µg. Block: 5% Skim Milk for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at room temperature. Color Development: ECL solution for 6 min at room temperature. Predicted/Observed Size: ~100kDa.

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Figure 3: Immunofluorescence - Anti-UVRAG Antibody (A305065)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-UVRAG Antibody (A305065), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UVRAG Antibody (D) Composite.