This antibody detects ubiquitined proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.
Applicazioni
WB, ELISA
Diluizioni consigliate
WB: 1:1,000, ELISA: 1:1,000
Reattività
Species Independent
Immunogeno
Human Ubiquitin conjugated lysozyme.
Specie ospite
Rabbit
Clonalità
Monoclonal
Clona ID
RGL3R
Isotipo
IgG
Coniugare
Unconjugated
Purificazione
Protein A purification.
Concentrazione
1 mg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Conservazione
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Figure 1: Western Blot - Anti-Ubiquitin Antibody [RGL3R] (A305235)
Western blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Anti-Ubiquitin Antibody [RGL3R] (A305235) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Rabbit IgG:HRP at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: Multiple.
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