Anti-UBE3A Antibody (A13635)

Name: Anti-UBE3A Antibody
Brand: Antibodies.com
SKU: A13635
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to UBE3A for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-UBE3A Antibody

Description

Rabbit polyclonal antibody to UBE3A.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-590 of human UBE3A (NP_570853.1).

Sequence

KLIRLWSKYNADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLKMVYYANVVGGEVDTNHNEEDDEEPIPESSELTLQELLGEERRNKKGPRVDPLETELGVKTLDCRKPLIPFEEFINEPLNEVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQGQQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPDIGMFTYDESTKLFWFNPSSFETEGQFT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

100 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

E6AP, E6AP ubiquitin-protein ligase, EPVE6AP, HECT-type ubiquitin transferase E3A, HPVE6A, Human papillomavirus E6-associated protein, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY-REN-54, Ubiquitin-protein ligase E3A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-UBE3A Antibody (A13635)

Western blot analysis of various lysates, using Anti-UBE3A Antibody (A13635) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-UBE3A Antibody (A13635)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-UBE3A Antibody (A13635) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-UBE3A Antibody (A13635)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-UBE3A Antibody (A13635) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-UBE3A Antibody (A13635)

Immunofluorescence analysis of HeLa cells using Anti-UBE3A Antibody (A13635) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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