Anti-UBE2C Anticorpo (A83441)

Name: Anti-UBE2C Antibody
Brand: Antibodies.com
SKU: A83441
Price: 480.0 USD
Availability: InStock

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$480

Anticorpo policlonale di capra contro UBE2C per ELISA, WB, IF, Flow Cytometry e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-UBE2C Antibody

Descrizione

Goat polyclonal antibody to UBE2C.

Applicazioni

ELISA, WB, IF, Flow Cytometry, IP

Diluizioni consigliate

ELISA: 1:32,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the C terminal region of human UBE2C.

Sequenza

C-QETYSKQVTSQEP

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

19 kDa

MW previsto

19.7kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

Cyclin selective ubiquitin carrier protein, dJ447F3.2, Mitotic specific ubiquitin conjugating enzyme, UB E2C, UBCH 10, UbcH10, UBE 2C, UBE2C_HUMAN, Ubiquitin carrier protein C, Ubiquitin carrier protein E2 C, Ubiquitin carrier protein E2C, Ubiquitin conjugating enzyme E2 C, Ubiquitin conjugating enzyme E2C, Ubiquitin protein ligase C, Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (4)

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Figure 1: Western Blot - Anti-UBE2C Antibody (A83441)

UBE2C expression in HEK293 (A) and HeLa (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-UBE2C Antibody (A83441) at 2µg/ml and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-UBE2C Antibody (A83441)

UBE2C expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-UBE2C Antibody (A83441) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic/plasma membrane staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

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Figure 3: Immunofluorescence - Anti-UBE2C Antibody (A83441)

UBE2C expression in MCF7 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-UBE2C Antibody (A83441) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

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Figure 4: Flow Cytometry - Anti-UBE2C Antibody (A83441)

UBE2C expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-UBE2C Antibody (A83441) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.