Sheep polyclonal antibody to Tyrosine Hydroxylase.
Specificità
This antibody recognises rat tyrosine 3-monooxygenase, also known as tyrosine hydroxylase or tyrosine 3-hydroxylase. Tyrosine hydroxylase is a 497 amino acid ~56 kDa enzyme involved in the production of dopamine from L-tyrosine. Tyrosine hydroxylase also catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons.
Applicazioni
WB, IHC-Fr, IF
Diluizioni consigliate
IF: 1:1,000, IHC-Fr: 1:1,000, WB: 1:1,000
Reattività
Rat, Mammals, Rabbit
Immunogeno
Rat Tyrosine hydroxylase purified from pheochromocytoma.
Specie ospite
Sheep
Clonalità
Polyclonal
Isotipo
IgG
Coniugare
Unconjugated
Concentrazione
Lot Specific
Peso molecolare
Approximately 60 kDa in rat caudate cell lysates.
Forma del prodotto
Liquid
Formulazione
Supplied in 10mM HEPES, pH 7.5, with 150mM NaCl, 0.01% BSA, 50% Glycerol, and 0.05% Sodium Azide.
Conservazione
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Note generali
Sheep anti Rat tyrosine hydroxylase antibody recognizes rat tyrosine 3-monooxygenase, also known as tyrosine hydroxylase or tyrosine 3-hydroxylase. Tyrosine hydroxylase is a 497 amino acid ~56 kDa enzyme involved in the production of dopamine from L-tyrosine. Tyrosine hydroxylase also catalyzes the rate-limiting step in the biosynthetic pathway of the catecholamines norepinephrine, and epinephrine. The enzyme exists as a tetramer, with each subunit composed of an N-terminal regulatory domain and a C-terminal catalytic domain. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons.
