This antibody recognises tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution.Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.

Applicazioni

WB, ELISA

Diluizioni consigliate

ELISA: 1:50 - 1:500, WB: 1:250 - 1:1,000

Reattività

Porcine, Mouse, Rat, Human

Immunogeno

Purified porcine brain tubulin.

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Concentrazione

5 mg/ml

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Conservazione

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Note generali

Rabbit anti Porcine tubulin antibody recognizes tubulin, a protein which is the major constituent of microtubules. Tubulin is a dimer of alpha and beta chains, which binds two molecules of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. There are at least six isotypes of both alpha- and beta- tubulin in human cells, which are distinguished by slightly different amino acid sequences and encoded by a large, multigene family that has been highly conserved throughout evolution. Although the most important functions of microtubules in proliferative cells are through their actions as components of the mitotic spindle, they are also involved in many other essential functions throughout the cell cycle of both malignant and nonmalignant cells. Antimicrotubule agents including Vinca alkaloids and taxanes may disrupt many of these essential functions.

Sinonimi

Alpha tubulin, Alpha-tubulin ubiquitous, beta Ib tubulin, CDCBM5, CDCBM6, fd02b12, K ALPHA 1, M40, OK/SW-cl.56, TBA1B_HUMAN, TUBA1B, TUBB1, TUBB2, TUBB5, tubulin alpha 1b, Tubulin alpha-1 chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-ubiquitous chain, Tubulin beta, Tubulin beta 1b, Tubulin beta 2A, tubulin beta 2A class IIa, Tubulin beta 2b, tubulin beta chain, tubulin beta class I, tubulin beta-1 chain, tubulin beta-2A chain, tubulin beta-5 chain, Tubulin K-alpha-1, tubulin, alpha, ubiquitous, tubulin, beta 2A class IIa, tubulin, beta polypeptide, tubulin, beta polypeptide 2, tubulin, beta, class IIA, wu:fd02b12, zgc:55461

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.