Figure 1: Western Blot - Anti-Triosephosphate Isomerase Antibody (A83170)
Triosephosphate Isomerase expression in HepG2 (A), HEK293 (B), HeLa (C), and Jurkat (D) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Triosephosphate Isomerase Antibody (A83170) at 0.001µg/ml and detected by chemiluminescence.
Figure 2: Western Blot - Anti-Triosephosphate Isomerase Antibody (A83170)
Triosephosphate Isomerase expression in RAW2647 (A), KNRK (B) and MDCK (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Triosephosphate Isomerase Antibody (A83170) at 0.001µg/ml and detected by chemiluminescence.
Triosephosphate Isomerase expression in Human Liver analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in Tris/EDTA buffer, pH 9. Staining was performed with Anti-Triosephosphate Isomerase Antibody (A83170) at 2µg/ml and revealed with horseradish peroxidase (HRP).
Triosephosphate Isomerase expression in Human Pancreas analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in Tris/EDTA buffer, pH 9. Staining was performed with Anti-Triosephosphate Isomerase Antibody (A83170) at 5µg/ml and revealed with alkaline phosphatase (AP).
Triosephosphate Isomerase expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Triosephosphate Isomerase Antibody (A83170) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
