Anti-TRIM21 Anticorpo (A83914)

Name: Anti-TRIM21 Antibody
Brand: Antibodies.com
SKU: A83914
Price: 480.0 USD
Availability: InStock

Questo prodotto è identico a Abcam (ab4369). Scopri di più con meno.

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$480

Anticorpo policlonale di capra contro TRIM21 per ELISA, WB, IF e Flow Cytometry.

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Consegna

Spese di spedizione

$40

Tempi di consegna

3-5 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanzie

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Descrizione Prodotto

Nome

Anti-TRIM21 Antibody

Descrizione

Goat polyclonal antibody to TRIM21.

Applicazioni

ELISA, WB, IF, Flow Cytometry

Diluizioni consigliate

ELISA: 1:16,000, WB: 0.2-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reattività

Human

Immunogeno

Synthetic peptide corresponding to the C terminal region of human TRIM21.

Sequenza

CPLNIGSQGSTDY

Specie ospite

Goat

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentrazione

500 µg/ml

Peso molecolare

60 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Sinonimi

52 kDa ribonucleoprotein autoantigen Ro/SS-A, 52 kDa Ro protein, 52kD Ro/SSA autoantigen, Autoantigen Ro/SSA, 52-KD, E3 ubiquitin-protein ligase TRIM21, RING finger protein 81, RNF81, Ro 52, Ro(SS-A), Ro52, RO52_HUMAN, Sicca syndrome antigen A, Sjoegren syndrome type A antigen, Sjogren syndrome antigen A1, Sjogren syndrome type A antigen, SS-A, SSA, SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro), Tripartite motif protein TRIM21, Tripartite motif-containing 21, Tripartite motif-containing protein 21

Controlli isotipo

Goat IgG (A121671)

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (3)

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Figure 1: Western Blot - Anti-TRIM21 Antibody (A83914)

TRIM21 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress MYC tagged TRIM21 an lysed in RIPA buffer. Primary incubation was performed on 10µg protein per lane with Anti-TRIM21 Antibody (A83914) at 1µg/ml or Anti-MYC at 1:1000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-TRIM21 Antibody (A83914) at 1mg/ml (Lane B).

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Figure 2: Immunofluorescence - Anti-TRIM21 Antibody (A83914)

TRIM21 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRIM21 Antibody (A83914) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Some cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-TRIM21 Antibody (A83914)

TRIM21 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-TRIM21 Antibody (A83914) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.