Recombinant human monoclonal [PY159] antibody to TREM1.
Applicazioni
ELISA, FACS, Functional Assay, In Vivo
Reattività
Human
Specie ospite
Human
Clonalità
Monoclonal
Clona ID
PY159
Isotipo
IgG1
Catene leggere
kappa
Coniugare
Unconjugated
Purificazione
Protein A affinity chromatography.
Concentrazione
Reconstitution dependent.
Peso molecolare
This antibody has a predicted MW of 143.66 kDa.
Purezza
> 95% (by SDS-PAGE and SEC-HPLC).
Forma del prodotto
Lyophilized
Ricostituzione
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulazione
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Conservazione
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Anti-TREM1 Antibody [PY159] - Low endotoxin, Azide free (A324278) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
Human TREM1 HEK293 cells were stained with Anti-TREM1 Antibody [PY159] - Low endotoxin, Azide free (A324278) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.7744nM.
The endocytosis ratio of Anti-TREM1 Antibody [PY159] - Low endotoxin, Azide free (A324278) by hu-TREM1-HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 80% at antibody concentration of 0.3 nM.