Mouse monoclonal [MEM-75] antibody to Transferrin Receptor (Biotin).
Specificità
This antibody reacts with an extracellular epitope of CD71 antigen, a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and T lymphocytes, macrophages, and erythroid precursors. It is lost on resting blood leukocytes. This antibody does not block binding of transferrin to the receptor.
Applicazioni
Flow Cytometry, IP, ICC
Diluizioni consigliate
Flow Cytometry: 2-5 µg/ml.
Reattività
Human
Immunogeno
NALM-6 human pre-B cell line.
Specie ospite
Mouse
Clonalità
Monoclonal
Clona ID
MEM-75
Isotipo
IgG1
Coniugare
Biotin
Purificazione
This antibody is conjugated with Biotin LC-NHS ester under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free Biotin.
Concentrazione
1 mg/ml
MW previsto
95 kDa
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cells stained with Anti-CD71 Antibody [MEM-75] (Biotin) (concentration in sample is 0.6 µg/ml, Streptavidin-APC).
Separation of cells stained with Anti-CD71 Antibody [MEM-75] (Biotin) (concentration in sample is 0.6 µg/ml, Streptavidin-APC, red-filled) from cells unstained by primary antibody (Streptavidin-APC, black-dashed) in flow cytometry analysis (surface staining).