This Total Antioxidant Capacity Assay Kit is a quantitative assay kit for measuring Total Antioxidant Capacity in serum, plasma, animal and plant tissues, cells, bacteria, and urine.

Applicazioni

Functional Studies

Principio di kit

This kit provides a convenient tool for detection of total antioxidant capacity in serum, plasma, animal and plant tissues, cells, bacteria, urine, and other biological fluids. The principle is that in an acidic environment, antioxidants can reduce Fe3+-Tripyridine triazine (Fe3+-TPTZ) to produce blue Fe2+-TPTZ. The total antioxidant capacity of samples is obtained by determining the content of Fe2+-TPTZ at 593 nm. Do not use EDTA as the anticoagulant for serum and plasma samples. The samples also can not contain DTT, Mercaptoethanol, Tween or Triton, or NP-40.

Tipo di campione

Serum, plasma, animal tissues, plant tissues, cells, bacteria, and urine.

Componenti

Assay Buffer (10X), Substrate Diluent, Substrate, Reaction Buffer, Ascorbic acid (Positive Control)

Conservazione

Shipped at 4°C. Store at -20°C in the dark for up to 12 months from receipt. Refer to the Protocol Manual for storage conditions of individual components. Do not use past expiration date!

Note generali

96-well microplate / microglass cuvette is required.

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (1)

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Figure 1: Standard Curve - Total Antioxidant Capacity Assay Kit (A319748)

Representative standard curve for Total Antioxidant Capacity Assay Kit (A319748). The y-axis is OD593 and the x-axis is Fe2+ concentration (mM).

Consegna

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Garanzie

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