Anti-Topoisomerase I Anticorpo [ARC0708] (A307759)

Name: Anti-Topoisomerase I Antibody [ARC0708]
Brand: Antibodies.com
SKU: A307759
Price: 470.0 USD
Availability: InStock

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$470

Anticorpo monoclonale [ARC0708] di coniglio contro Topoisomerase I per WB, IHC, ICC/IF e IP.

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Consegna

Spese di spedizione

$40

Tempi di consegna

5-8 Giorni lavorativi

Contattaci

Telefono

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Descrizione Prodotto

Nome

Anti-Topoisomerase I Antibody [ARC0708]

Descrizione

Rabbit monoclonal [ARC0708] antibody to Topoisomerase I.

Applicazioni

WB, IHC, ICC/IF, IP

Diluizioni consigliate

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reattività

Human, Mouse, Rat

Immunogeno

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (TOP1) (P11387).

Sequenza

MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDA

Specie ospite

Rabbit

Clonalità

Monoclonal

Clona ID

ARC0708

Isotipo

IgG

Coniugare

Unconjugated

Purificazione

Affinity purification.

Peso molecolare

100 kDa

Forma del prodotto

Liquid

Formulazione

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Conservazione

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Sinonimi

DNA topoisomerase 1, DNA topoisomerase I, NUP98 fusion gene, TOP 1, TOP I, TOP1, TOP1_HUMAN, TOPI, Topoisomerase (DNA) I, Topoisomerase 1, Topoisomerase1, TopoisomeraseI, Type I DNA topoisomerase

Controlli isotipo

Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.

Immagini (7)

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Figure 1: Western Blot - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Western blot analysis of extracts of various cell lines, using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Western blot analysis of extracts of Mouse thymus, using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 3: Immunohistochemistry - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Topoisomerase I Antibody [ARC0708] (A307759)

Immunofluorescence analysis of C6 cells using Anti-Topoisomerase I Antibody [ARC0708] (A307759) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).