Figure 1: Western Blot - Anti-TMS1/ASC Antibody (Biotin) (A83387)
Anti-PYCARD Antibody (A83387 | Biotin) (3µg/ml) staining of U937 lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
