Mouse monoclonal [HTA125] antibody to TLR4 (Biotin).
Specificità
This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen.TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule.TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL ) is recommended for functional assays.
Applicazioni
Flow Cytometry
Diluizioni consigliate
Use 10µl of the suggested working dilution to label 106 cells or cells or 100µl whole blood
Protein G affinity chromatography of tissue culture supernatant.
Concentrazione
100 µg/ml
Forma del prodotto
Liquid
Formulazione
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Conservazione
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Note generali
Mouse anti Human CD284 antibody, clone HTA125 recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. The use of a preservative free format of Mouse anti Human CD284 antibody, clone HTA125 (MCA2061EL ) is recommended for functional assays.
Sinonimi
ARMD10, CD284, CD284 antigen, Homolog of Drosophila toll, hToll, TLR 4, TLR4_HUMAN, TOLL, Toll like receptor 4, Toll-like receptor 4
