This antibody recognises human TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, which is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts. TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors. A correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth

Applicazioni

WB, ELISA, IHC-P, Functional Assays

Diluizioni consigliate

ELISA: 0.5 - 2 µg/ml, WB: 0.1 - 0.2 µg/ml

Reattività

Human

Immunogeno

Recombinant human TIMP-1.

Specie ospite

Rabbit

Clonalità

Polyclonal

Isotipo

IgG

Coniugare

Unconjugated

Concentrazione

1 mg/ml after reconstitution.

Forma del prodotto

Lyophilized

Ricostituzione

Reconstitute with 0.1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Formulazione

Supplied in Phosphate Buffered Saline.

Conservazione

Lyophilized: Store at -20°C. Reconstituted: Store undiluted at -20°C, avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Note generali

Rabbit anti Human TIMP-1 antibody recognizes human TIMP-1 (Tissue Inhibitor of Metalloproteinase -1), a secreted glycoprotein and member of the protease inhibitor I35 (TIMP) family, which is expressed by a wide range of cells, including microvascular endothelial cells, smooth muscle cells, mesangial cells, and some fibroblasts. TIMP-1 acts as a natural extracellular inhibitor of MMPs (matrix metalloproteinases), a group of peptidases involved in degradation of the extracellular matrix, and is also involved in erythropoiesis, through the simulation of the proliferation/differentiation of erythroid progenitors. A correlation between localized concentrations of TIMP-1 and TIMP-3, may determine cell fate, and that increased expression of TIMP-1, may act as an inhibitor/suppressor of invasive cell growth (Sternlicht and Werb 2001)

Sinonimi

Clgi, Collagenase inhibitor, Collagenase inhibitor, Human, EPA, EPO, Erythroid Potentiating Activity, Erythroid-potentiating activity, Fibroblast collagenase inhibitor, FLJ90373, HCI, Human Collagenase Inhibitor, Metalloproteinase inhibitor 1, Metalloproteinase inhibitor 1 precursor, OTTHUMP00000023214 , TIMP, TIMP 1, TIMP metallopeptidase inhibitor 1 , TIMP-1, TIMP1 protein, TIMP1_HUMAN, Tissue Inhibitor of Metalloproteinase 1, Tissue inhibitor of metalloproteinases, Tissue inhibitor of metalloproteinases 1, Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor

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Secondari adatti

Dichiarazione di non responsabilità

Questo prodotto è solo per uso di ricerca. Non è inteso per uso diagnostico o terapeutico.