Figure 1: Western Blot - Anti-Thyroid Peroxidase/TPO Antibody (Biotin) (A83131)
Anti-TPO Antibody (A83131 | Biotin) (1µg/ml) staining of Human Thyroid Gland lysate (35µg protein in RIPA buffer), exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.